Human IgG Fc/Fab Medium Titer Assay (PA 101-01/10)

This assay covers IgG concentrations which are typical for clone screenings of CHO cell after single cell seeding and cultivation periods of 10−14 days in 96- or 384-well plate formats. It uses ProteinA coated capture beads and a fluorescence marker against the Fab part of human IgG in a direct assay format.

The sample throughput of this assay can be greatly improved if multiple plates are incubated in parallel. This allows for the screening of thousands of clones per day.

The plate can also be partially used and remaining wells can be used later.

One assay kit contains one 384-well PAIAplate with pre-dispensed dried capture beads and 25 mL ready-to-use assay buffer.

Assay characteristics:

  • Analytes: human IgG1, IgG2 and IgG4
  • Direct assay format
  • Concentration range: 5−200 µg/mL IgG*
  • Sample volume: 6 µL undiluted crude sample
  • 60 min total assay time/384-well plate
  • Clone screening
  • Wavelengths: Ex. 640nm/Em. 665nm

* The concentration range has been determined in accordance with the EMEA guideline on the validation of bioanalytical methods.


Related documents

PA-101 user manual huIgG Fc-Fab_medium titer.pdf • 927 kB

PA-101 short instruction.pdf • 893 kB

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