Our special PAIAplates are based on the SBS 384-well standard format. They have been optimized for the efficient and complete separation of the capture beads and for sensitive detection of fluorescence in the assay solution. Each well contains a transparent pyramidal structure in the middle of a black well bottom on which the capture beads settle once the assay is terminated. The black bottom prevents the beads from being measured by fluorescence bottom reading.
The state-of-the-art manufacturing process uses highly transparent material with low autofluorescence to generate 384 highly uniform structures on the bottom of each well in a PAIAplate to ensure high assay reproducibility. The size of the structures allows efficient mixing of the assay components during the incubation and creates a sufficiently large detection window for standard measurement devices like plate readers and fluorescence microscopes.
The PAIAplates are measured on standard microplate fluorescence readers with a bottom-read option. It is also possible to use automated inverted fluorescence microscopes (e.g. Cellavista or NyONE from SynenTec GmbH ) with very similar performance.
An orbital shaker with sufficient speed is required to efficiently incubate the assay components (typically at 1500−2000 rpm). The use of multiple shakers in parallel enhances the throughput dramatically, because the incubation is the most time consuming assay step.