384 individual beads-based assays can be performed in parallel on a single PAIAplate.
The kits contain plates that are pre-loaded with dried capture beads and come with reagents for the detection of the analyte in the sample by fluorescence.
PAIA assays are completely plate-based and only require the addition of reagent & sample.
Washing steps are eliminated and bead separation is achieved by simple sedimentation that removes the beads from the light path.
We have reinvented the way bead-based fluorescence assays have been traditionally measured. Instead of detecting the amount of fluorescence bound to the beads which requires separation and washing of the beads, the PAIAplate allows direct measurement of the fluorescence of the free reagent that has not bound to the beads.
This fluorescence is inversely proportional to the fluorescence on the beads and can equally be used for quantitative measurements.
The PAIAplate is the key to our technology. Based on a common microplate format, it contains 384 black wells featuring a transparent pyramid in the middle.
This structure ensures that the sedimented beads are removed from the light path and only fluorescence from the solution in measured.
PAIAplates can be measured on common fluorescence plate readers and microscopes.
PAIA assays have been automated on liquid handlers from different manufacturers. A liquid handler for pipetting volumes in the range of 5 to 50 µL is required.
We can provide instructions to achieve the require dispensing precision. The use of multi-channel heads, especially 96-channels heads are recommended to increase the throughput.
This example shows the automation on an Assist Plus liquid handler (Integra Bioscience).
The PAIAplate is first loaded with reagent and then samples from a 96-well source plate are transferred
to the PAIAplate.
A pipet with variable spacing is used to conveniently place the 96 samples into one quadrant of the PAIAplate.